Mobile search icon
Herbs and spices >> Analyses >> Spices

Spice analysis

ISO Standard

For spices and herbs where the International Organization for Standardization (ISO) has developed a standard laying down specifications, the specific ISO standard is presented under each herb or spice.The purpose of ISO is to help unify standards on an international basis.

(Pimpinella anisum)

ISO 7386:1984 Aniseed (Pimpinella anisum Linnaeus)

Aniseed (Pimpinella anisum Linnaeus) has long been popular because of its many uses: culinary, household and cosmetic amongst others. It is a flowering plant native to the eastern Mediterranean region and South-West Asia. It is the fruit (seed) or the anise oil that is of main comersial interest.

Quality          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber  LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  IDA42 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /10g 8
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
Tropane alkaloids JCTA3 Internal Method  LC-MS/MS 5µg/kg 9
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Piper Nigrum)

ISO 959-1:1998 Pepper (Piper nigrum L.), whole or ground - Specification Part 1: Black pepper
ISO 959-2:1998 Pepper (Piper nigrum L.), whole or ground - Specification Part 2: White pepper

Black pepper, (P. nigrum), also known as the king of spices, belongs to the family Piperaceae, is cultivated for its fruit, which is usually dried, and used as a spice and seasoning. Black pepper has multiple uses in the processed food industry, in the kitchen, in perfumery, traditional medicine and even in beauty care. Pepper is valued for its pungency and flavour, which is attributed by the alkaloid piperine and the volatile oil.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter (whole, crushed) IDA39 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /100g 8
Extraneous matter (ground) IDA38  AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /50g 8
Piperin J3178 ASTA 12.1:1997-01, mod. Spectrophotometry 0,1% 12
Bulk density  EF145 Internal Method  Gravimetry g/ml 7
Light Berries in Whole Black or White Pepper  QP005 ASTA 14.2 Microscopy % 12
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  PJKPL DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Calcium SL03C/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 20 mg/kg 8
Copper SL03H/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,05 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
Soy LW0PE Elisa Systems ELISA 2,5 mg/kg 7
Sesame LW1JG SENSISpec ELISA 2 mg/kg 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations
The most common adulterants used in whole or ground pepper are low quality pepper and various foreign substances. What is also seen are counterfeits with stalks, chaff or similar organic foreign material. When adulterated with other species, chili, buckwheat, millet or papaya have been observed.

Microbiology
           
           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

Capsicum annuum and Capsicum frutescens

ISO 7540:2020 Ground sweet and hot paprika (Capsicum annuum L. and Capsicum frutescens L.)
ISO 972:1997 Chillies and capsicums, whole or ground (powdered)

Capsicum are widley grown for their fruit that may be eaten raw, cooked and used as dried powder. Many different varieties of the genus Capsicum exist and the nomenclature has a rather national naming structure spread over the world i e the name Paprika can have a different meaning in different countries. Capsicum is importent in trade and is one of the first spices used by human. Capsicum are native to Mexico and Central America but are grown withspread all over the world.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber  LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Colour value LYCA1 ASTA 20.1:2004-10 Spectrophotometry - 12
Capsaicin J8022/JJ0BX ASTA 21.3:2004-10, mod. Chromatography 20 mg/kg Ts 9
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Alternaria toxins JCW8H Internal Method  LC-MS/MS 10-50 µg/kg 9
Irradiation  PJKPL DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Illegal dyes JCFS3 Internal Method  LC-MS/MS 10-100 µg/kg 7
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
Almond LW18G r-Biopharm ELISA 2,5 mg/kg 7
Peanut LW186 Neogen Veratox ELISA 2,5 mg/kg 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations          
Several types of adulteration are possible and have been reported. Whole dried chillies may be adulterated by adding sub-standard dried fruits, which are darkened or bleached, by adding moisture to increase the weight of a load, or by coating with mineral oil or synthetic dyes such as coal tar red to improve the colour and appearance.  A more difficult problem is the addition of varieties or harvests that vary in CAPS and colour but have similar shape, size, and visual colour. Chilli powder may be adulterated by adding extra amounts of bleached pericarp, seeds, calyx, and peduncle to increase bulk without visibly affecting appearance. Other reported adulterants include artificial dyes and/or illegal (sudan), almond shell dust, and dried red beet pulp.
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Carum carvi)

 ISO 5561:1990 Black Caraway and Blond Caraway (Carum carvi Linnaeus),whole

Caraway, also known as meridian fennel and Persian cumin (Carum carvi), is a biennial plant in the parsley family Apiaceae, native to western Asia, Europe, and North Africa and cultivated since ancient times. Caraway has a distinctive aroma reminiscent of anise and a warm, slightly sharp taste.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  ID0GC AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /10g 8
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Copper SL03H/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,05 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg7kg 3
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Elettaria Cardamomum)

ISO 882-1:1993 Cardamom (Elettaria cardamomum (Linnaeus) Maton var. minuscula Burkill) — Specification — Part 1: Whole capsules
ISO 882-2:1993 Cardamom (Elettaria cardamomum (Linnaeus) Maton var. minuscula Burkill) — Specification — Part 2: Seeds

Small cardamom (green cardamom), known as the ‘queen of spices’, belongs to the family of ginger and turmeric ( Zingiberaceae), is a rich spice obtained from the seeds of a perennial plant, Elettaria cardamomum Maton. It is one of the highly prized spices of the world and is the third most expensive spice after saffron and vanilla.
There is also Black cardamom or large cardamom (Amonum subulatum), Though the fruits are clearly identifiable by their larger size and differences in shapes compared with small cardamom, the seeds are of nearly the same size as those of true cardamom but has a different flavour. Similar use as green cardamom except for sweet dishes. Mainly used in Asia. Black cardamom is covered in ISO 10622:1997

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber  LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  IDA42 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /10g 8
           
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Calcium SL03C/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 20 mg/kg 8
Copper SL03H/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,05 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
           
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
Peanut LW186 Neogen Veratox ELISA 2,5 mg/kg 7
Sesame LW1JG SENSISpec ELISA 2 mg/kg 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulteration          
Decorticated seeds can be adulterated with seeds from lower grades and also from large cardamom as they are of similar shape, size and colour. Pale brown coloured seeds would represent immature cardamom which are low in volatiles and poor in quality and intensity. The seeds from large cardamom have lower volatile oil content, entirely different composition and aroma. 
Adulteration of cardamom powder is possible with almost any material powdered to similar size. Cereal and pulse flours and extracted ginger have been reported as adulterants.   
           
Microbiology          
Analysis Test code Method reference Analytical technique Limit (ESA) Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Apium graveolens)

ISO 6574:1986 Celery seed (Apium graveolens Linnaeus)

Celery, Apium graveolens is a biennial plant that occurs around the globe. Celery grows wild in Europe, the Mediterranean region and in Asia. It was probably first grown as a medicinal plant, later for the leaves and stem as flavouring

Quality   
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  IDA42 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /10g 8
           
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method P-14.194-6 GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS  0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
           
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Cinnamomum zeylanicum)

ISO 6539:2014 Cinnamon (Cinnamomum zeylanicum Blume)

Cinnamon and its close relative, cassia, are among the earliest, most popular spices used by mankind. The cinnamon of commerce is the dried inner bark of the tree, C. verum or Cinnamomum zeylanicum. While cinnamon and cassia are not precisely the same, they are closely related and the bark of the two is simiar. The true cinnamon, Cinnamomum verum syn. C. zeylanicum, is native of Sri Lanka and South India. Cassia is covered by ISO 6538:1997

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Volatile oil LW2Q8 ASTA 16.0:2013 mo Volumetry 0,3 ml/100g 7
Extraneous matter  IDA43 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /50g 8
Coumarin JKCUG § 64 LFGB L 00.00-134:2010-09, mod. LC-MS/MS 20 mg/kg 9
         
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
Illegal dyes JCFS3 Internal Method  LC-MS/MS 10-100 µg/kg 7
Griseofulvin SFULV §64 LFGB 00.00-115:2018:10 (QuEChERS) mod. LC-MS/MS 0,01 mg/kg 9
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
Peanut LW186 Neogen Veratox ELISA 2,5 mg/kg 7
Sulphite JJ009 § 64 LFGB L 00.00-46/1, mod:1999-11, mod. Titrimetry 5 mg/kg 9
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier
           
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations          
Cinnamon is frequently adulterated with a rougher thicker and less aromatic bark from cassia and C. tamala. Bark powder can be adulterated with powdered beechnut husks, aromatised with cinnamaldehyde. Other adulteration that has been detected is with sugar, ground walnut shells, coffe husk, galanga rhizome. Color enhancement and weight increase has been seen with lead chromate.
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Syzygium aromaticum)

ISO 2254:2004 Cloves, whole and ground (powdered)

Cloves are the aromatic flower buds of an evergreen tree in the family Myrtaceae, Syzygium aromaticum. Cloves were important in the earliest spice trade and are believed to be indigenous to the Moluccas, or Spice Islands, of Indonesia. Strong of aroma and hot and pungent in taste cloves are used to flavor a wide variety of foods.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  ID0GC AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /25g 8
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A A7126/JJP01 DIN EN 14132 (2009-09), mod. LC-FLD 0,5 µg/kg 7
Irradiation  PJKPL DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
           
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Cuminum cyminum)

ISO 6465:2009 Cumin (Cuminum cyminum L.) 

Cumin is the dried seed like fruit of the herb Cuminum cyminum, a member of the parsley family. Native to the Mediterranean region, also cultivated in India, China, and Mexico and is used to flavour a variety of foods.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber  LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  ID0GC AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /25g 8
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
Soy LW0PE Elisa Systems ELISA 2,5 mg/kg 7
Sesame LW1JG SENSISpec ELISA 2 mg/kg 7
Hazelnut LW0Wt Elisa Systems ELISA 0,5 mg/kg 7
Almond - Elisa LW18G r-Biopharm ELISA 2,5 mg/kg 7
Peanut-Elisa LW186 Neogen Veratox ELISA 2,5 mg/kg 7
Almond - PCR JKPAL/J6000 § 64 LFGB L 18.00-20:2014-08, mod. Real-time PCR Detected/Not detected 7
Peanut-PCR JKPPN  64 LFGB L 00.00-169:2019-07, mod. Real-time PCR Detected/Not detected 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations 
Presence of mahaleb, almond, almond husks, peanut, peanut shells, tree nuts, peach and cherry, fennel seeds or fennel seeds covered with marble dust and dye.

           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Foeniculum vulgare)

ISO 7927-1:2023 Fennel seed, whole or ground - Part 1: Bitter fennel seed specification (Foeniculum vulgare P. Miller var. vulgare)
ISO 7927-2:2023 Fennel seed, whole or ground - Part 2: Sweet fennel seed specification (Foeniculum vulgare var. panmorium)

Fennel (Foeniculum vulgare) Is native to southern Europe and the Mediterranean region but is widely cultivated, both in its native habitat and elsewhere, for its edible, strongly flavoured leaves and seeds.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  ? AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /25g 8
           
           
Contaminants/Unwanted substances      
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Trigonella foenum-graecum)

ISO 6575:1982 Fenugreek, whole or ground (powdered)

Fenugreek (Trigonella foenum-graecum) fragrant herb of the pea family Fabaceae, its dried flavourful seeds used as a spice. It is cultivated worldwide as a semiarid crop. Its seeds and leaves are common ingredients in dishes from the Indian subcontinent, and have been used as a culinary ingredient since ancient times.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. Photoluminicence  depending on substance 4
Ethylene oxide ZPET0 Internal Method  LC-MS/MS, GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. GC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
Pyrrolizidine alkaloids  JCPAS LC-MS/MS LC-MS/MS 5 µg/kg 9
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Gluten LW23R SENSISpec Ingezim ELISA - 3
Soy LW0PE Elisa Systems ELISA 2,5 mg/kg 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Allium sativum)

ISO 5560:1997 Dehydrated garlic

Garlic (Allium sativum L.) is the second most widely cultivated Allium after onion. Garlic is in demand almost all the year round all over the world both in fresh and dehydrated form. Today, garlic is used for its flavour, aroma and taste being prepared domestically or forming a raw material for a variety of food manufacturing processes (dehydration and pickling). Alliums have been cultivated for thousands of years for therapeutic and prophylactic properties, religious significance, flavour and taste. Garlic is thought to have originally come from Central Asia and Southern Europe, especially the Mediterranean region.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber  LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Starch MJ011 Internal Method  Spectrophotometry % 9
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter  IDA45 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /50g 8
Allin YP9GX Internal Method  LC-UV 50mg/100g 17
Sulfur SL0AE/SLC11 SS-EN ISO 11885:2009 / SS-EN 13805:2014 mod. ICP-MS 50mg/kg 8
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  PJKPL DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Calcium SL03C/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 20 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Mustard LW18G Neogen Veratox ELISA 2,5 mg/kg 7
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
Peanut LW186 Neogen Veratox ELISA 2,5 mg/kg 7
Sesame LW1JG SENSISpec ELISA 2 mg/kg 7
Sulphite JJ010 ZLUF A 111(3), S.198-207:1959 Titrimetry 5 mg/kg 9
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Limit (ESA) Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Zingiber officinale Roscoe)

ISO 1003:2008 Ginger

Ginger, the rhizome of Zingiber officinale Roscoe, is a widely used spice. It is used fresh, preserved or in dried form. India and China are the world’s largest producers and exporters of ginger but also Jamaican, Indian and West Africa are producing countries. The aroma and flavour of fresh ginger will be different from dry ginger as some of the volatile oils will be lost by evaporation during drying.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Crude fiber  LP08B ISO 5498 mod. Gravimetry 0,5g/100g 7
Starch MJ011 Internal Method  Spectrophotometry % 9
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Gingerol/Shogaols M902Q Internal Method  LC-DAD µg/g 15
Extraneous matter  IDA42 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /10g 8
           
           
Contaminants/Unwanted substances      
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
Calcium SL03C/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 20 mg/kg 8
Copper SL03H/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
           
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Soy LW0PE Elisa Systems ELISA 2,5 mg/kg 7
Sesame LW1JG SENSISpec ELISA 2 mg/kg 7
Peanut LW186 Neogen Veratox ELISA 2,5 mg/kg 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
           
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Myristica fragrans)

ISO 6577:2002 Nutmeg, whole or broken, and mace, whole or in pieces

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
Extraneous matter (ground nutmeg) IDA48 AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /10g 8
Extraneous matter (whole nutmeg) QS05X ASTA 14.1 Microscopy - 14
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  PJKPL DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
Calcium SL03C/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 20 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Soy LW0PE Elisa Systems ELISA 2,5 mg/kg 7
Sesame LW1JG SENSISpec ELISA 2 mg/kg 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations         
Adulteration is common in the nutmeg trade. The essential oil has often been extracted before the nuts are marketed and such nuts can be detected by their light weight and are more subjected to insect attack. Also dilution with mutmeg pericarp, spent powder, coffe husk or similar product have been seen.
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Crocus sativus)


ISO 3632-1:2010 Saffron

Saffron, the most expensive spice in the world, is derived from the dry stigmata of the saffron crocus Crocus sativus L. Major saffron cultivating countries for trade are Spain, Iran, Greece, India, China and Morocco. Saffron is hand-harvested, still the only method for harvesting the crop.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LP210 ISO 3632-2 Gravimetry  g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,2 g/100g 7
Picrocrocin,  bitterness LP208 ISO 3632-2 Spectrophotometry - 9
Crocin, colouring strength LP207 ISO 3632-2 Spectrophotometry - 9
Safranal, taste LP209 ISO 3632-2 Spectrophotometry - 9
Artificial water-soluble acid colourants LW039 ISO 3632-2 TLC - 9
Ethereal extract LP206 ISO 941 Gravimetry g/100g Dm 9
*Minimum of 8g is needed to cover all analysis in tabel above      
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations           
Saffron of unknown origin labelled as being cultivated in the PDO region in Spain can be used for substitution. Other adulteration seen is color enhancement with illegal dyes, or substitution with other botanicals like pomegranate fibers, safflower, marigold, gardenia, wood powder, stigmas of other saffron types, flowers, starch, glucose.
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7

(Illicium verum)

ISO 5562:1983 Star anise

Star anise, is a medium-sized evergreen tree native to northeast Vietnam and southwest China. The spice that closely resembles anise in flavor is obtained from the star-shaped pericarps of the fruit of I. verum. The stars are available whole, or ground. Apart from its use in sweetmeats and confectionery, it contributes to meat and poultry dishes. The essential oil of star anise fruits is used in the confectionary trade to flavour liquorice and other sweets and in the baking trade to flavour cakes, cookies and biscuits. Japanese star anise (Illicium anisatum), a similar tree, is not edible because it is highly toxic and can cause neurological damage.

 

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Volatile oil LW022 ASTA 5.2:2010 mod. Volumetry 0,3 ml/100g 7
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 6
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
           
           
Allergens*          
Analysis Test code Method reference Analytical technique Limit (ISO) Response time (days)
Gluten LW23R SENSISpec Ingezim ELISA - 3
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Limit (ISO) Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations          
Chine star anise mixed with Japanese Star anise (poisonous)    
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5

(Curcuma longa)

ISO 5562:1983 Star anise (Illicium verum Hook. f.)

Turmeric, (Curcuma longa Linnaeus), is a perennial herbaceous plant of the ginger family (Zingiberaceae). The rhizomes are used fresh or boiled in water and dried, after which they are ground into a deep orange-yellow powder. Turmeric is used for vegetable, meat and soup-like dishes, used widely as a food colourant and is one of the principal ingredients in curry powder. There have also been an high interest in turmeric due to its medicinal properties. Turmeric is native to southern India and Indonesia, and is mainly grown in Asia.

Quality           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Water activity LW01F NMKL 168, 2001 Hygrometri - 3
Moisture LW01I ASTA Gravimetry 0,3 g/100g 7
Total ash LP06V NMKL 173 Gravimetry 0,1 g/100g 5
Acid-insoluble ash LP05A 2009/152/EU mod. Gravimetry 0,02 g/100g 7
Volatile oil LW022 ASTA Volumetry 0,3 ml/100g 7
Starch MJ011 Internal Method  Spectrophotometry % 9
Extraneous matter  ID0GC AOAC 970.66 1970 + AOAC 945.75 1996 Microscopy /25g 8
Curcumin LYCUR ASTA Analytical Method 18.0:2004-10 Spectrophotometry % 12
           
           
Contaminants/Unwanted substances        
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aflatoxins JJ006/JJP01 DIN EN 14123 (2008-03), mod. IAC-LC-FLD 0,1 µg/kg 9
Ochratoxin A LW0AC Ochraprep A3-P14.V1 LC-FLD 5 µg/kg 7
Irradiation  J3010 DIN EN 13751:2009-11 Photoluminicence  - 7
Pesticides (EC) 396/2005 PLW8L NMKL 195, 2013 mod. LC-MS/MS, GC-MS/MS depending on substance 4
Ethylene oxide ZPET0 Internal Method  GC-MS/MS 0,01 mg/kg 9
Chlorate/Perchlorate LW2AA QuPPe-PO-Method Ver 12 mod. LC-MS/MS 0,05 mg/kg 5
Nicotine JC016 Internal Method  LC-MS/MS 10 µg/kg 9
Lead SL03N/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,02 mg/kg 8
Cadmium SL03M/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 0,01 mg/kg 8
Calcium SL03C/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 ICP-MS 20 mg/kg 8
Nickel  SL0AD/SLC11 SS-EN ISO 17294-2:2016 /SS- EN 13805:2014 mod ICP-MS 0,05 mg/kg 8
PAH JC10U acc. EU 2005/108 GC-MS/MS 0,5 µg/kg 7
Mineral oil  JCMES Internal Method  LC-GC-FID 0,5-2mg/kg 7
Illegal dyes JCFS3 Internal Method  LC-MS/MS 10-100 µg/kg 7
Griseofulvin SFULV §64 LFGB 00.00-115:2018:10 (QuEChERS) mod. LC-MS/MS 0,01 mg/kg 9
           
           
Allergens*          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Gluten LW23R SENSISpec Ingezim ELISA 7 mg/kg 3
Soy LW0PE Elisa Systems ELISA 2,5 mg/kg 7
*Allergens above are only a suggestion: Also consider which food allergens your product may have come in contact with. Consider all possible steps like processing steps, transport, field contamination or co-handling by supplier.
           
Authenticity           
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
PCR NGS  BJNGP  Massive Parallel Sequencing PCR NGS - 16
NMR profiling AA01A 1H NMR spektroskopi NMR - 10
           
Known adulterations          
Whole, dried or fresh turmeric is usually free from adulteration. However, turmeric powder have been adulterated with foreign starch (tapioca, arrowroot, cereal floor), husks, coal tar chalk powder, colours like lead chromate etc. Also substitution with Curcuma zedoria or Curcuma malabarica has been seen.
           
Microbiology          
Analysis Test code Method reference Analytical technique Reporting limit Response time (days)
Aeroba microorganisms  UMH32 NMKL 86, 5. Ed., 2013 Microbiological cultivation 103 cfu/g 5
Enterobacteriaceae  ZM9ZS NMKL 144, 3. Ed., 2005 Microbiological cultivation 10 cfu/g 3
E.coli  UMR36 NMKL 125, 4. Ed., 2005 Microbiological cultivation 10 cfu/g 3
Bacillus cereus UM1TF NMKL 67, 8. Ed., 2021 Microbiological cultivation 102 cfu/g 3
Clostridium perfringens UM1UK NMKL 95, 5. Ed., 2009 Microbiological cultivation 102 cfu/g 3
Yeast and mould UMP74 NMKL 98, 4. Ed., 2005 Microbiological cultivation 102 cfu/g 7
Salmonella UML66 NMKL 71, 5. Ed., 1999 Microbiological cultivation absent in 25g 5